Born in Arcadia, California Rena Sofer grew up in Pittsburgh, Pennsylvania where she moved after her parents divorced.
At the age of 15 she was discovered by a New York talent scout and appeared in the TV series Another World for a short time before being cast as Amelia 'Rocky' McKenzie Domeq in the soapie Loving in 1988.
She made her film debut in 1992 in A Stranger Among Us, starring Melnaie Griffith and in 1993 she joined the cast of General Hospital as Lois Cerullo after moving from New York to Los Hospital.
She won a Daytime Emmy Award for the role and was noticed by Aaron Spelling who offered her the part of Eve Cleary in Melrose Place.
She went on to star with Ben Stiller and Edward Norton in the 2000 film Keeping the Faith and had a recurring role on ED as lawyer Bonnie Hayne (who was Ed's love interest). She played series regular Vicky Costa in 2002/2003 final season of Just Shoot Me and in 2005 she starred as Christie Dunbar in the crime series Blind Justice.